Human iPSC Core
The induced pluripotent stem cells (iPSC) core has set up an infrastructure of inter-institutional regulatory approvals to provide a number of human iPSC cell lines including parent and lineage and cell type specific reporter lines that can be differentiated into various kidney cell types. These cell lines are distributed throughout the world for various aspects of kidney development, disease modelling and tissue engineering.
JIT iPSC funding opportunity
PCEN offers Just in Time (JIT) funding for your iPSC and GTAC research. Take advantage of these programs now.
The details of how to request cell lines are provided at (Re)Building a Kidney consortium.
Summary of iPSC lines being distributed
| no. | Gene | Reporter | Targeting approach | Purpose | Parental line | Target validation | Genome integrity |
|---|---|---|---|---|---|---|---|
| 1 | Control | NA | NA | Parental | BJFF6 | Y | Y |
| 2 | Control | NA | NA | Parental | AN1 | Y | Y |
| 3 | Control | NA | NA | Parental | WTC11 | Y | Y |
| 4 | RET:AAVS1 | tdT:GCaMP6f | 5′, T2A | Collecting duct progenitors, UB:calcium sensor | WTC11 | Y | Y |
| 5 | WNT9B | mCerulean3 | 3′, T2A | UB stalk | BJFF6 | Y | Y |
| 6 | CA2 | E2 Crimson | 3′, T2A | Intercalated cells | BJFF6 | Y | Y |
| 7 | AQP2 | EGFP | 3′, T2A | Principal cells | BJFF6 | Y | Y |
| 8 | UPK3A | mCerulean3 | 5′, T2A | Ureter/bladder epithelium | BJFF6 | Y | Y |
| 9 | HCN3 | membranetdT | 3′, T2A | Pacemaker cells | BJFF6 | Y | Y |
| 10 | SIX2 | EGFP | 3′, T2A | nephron progenitors | CRL-2429 | Y | Y |
| 11 | CITED1 | mCherry | 3, T2A | nephron progenitors | CRL-2429 | Y | Y |
| 12 | GATA3 | mCherry | 5′, T2A | collecting duct | CRL-2429 | Y | Y |
| 13 | CUBN | EGFP | 5′, polyA | proximal tubule | CRL-2429 | Y | Y |
| 14 | MAFB | mTagBFP2 | 5′, polyA | podocytes | CRL-2429 | Y | Y |
| 15 | SIX2/ CITED1 | EGFP/ mCherry | As above | nephron progenitors | CRL-2429 | Y | Y |
| 16 | GATA3/ MAFB | mCherry/ mTagFBP2 | As above | collecting duct and podocytes | CRL-2429 | Y | Y |
| 17 | GAPDH | EGFPloxP mCherry | 3′, T2A | Lineage tracing tool | CRL-2429 | Y | Y |
| 18 | GAPDH/ SIX2 | EGFPloxP mCherry/ Cre | 3′, T2A 3, T2A | lineage tracing of nephron progenitors | CRL-2429 | Y | Y |
| 19 | GATA3/ CUBN/ MAFB | mCherry/ EGFP/ mTagBFP2 | As above | collecting duct, proximal tubule and podocytes | CRL-2429 | Y | Y |
| 20 | |||||||
| 21 | GAPDH/ SIX2 | EGFPloxP mCherry/ CreERT2 | 3′, T2A 3, T2A | Lineage tracing of nephron progenitors | CRL-2429 | Y | Y |
Director
Sanjay Jain, MD, PhD
Professor of Medicine, Nephrology
Professor of Pediatrics, Molecular Genetics and Genomics Program
Director Kidney Translational Research Center
Washington University in St. Louis School of Medicine
Sanjay Jain is a Professor of Medicine, Pediatrics and Pathology & Immunology at the Washington University School of Medicine in St. Louis, Missouri, USA (WUSM). His laboratory focuses on how kidneys and the lower urinary tract develop and organize to maintain homeostasis across lifespan in health and disease. His has defined key developmental pathways and mechanisms that regulate the joining of primitive ureter and bladder, initiation of the collecting system and branching morphogenesis of the kidney and genetic mutations associated with CAKUT. He leads multiple NIH-sponsored atlas efforts to map healthy and disease states in the human kidney including HuBMAP, KPMP, RBK/GUDMAP and Pediatric Center of Excellence in Nephrology. The team has identified, validated and mapped ~100 cell identities in the kidney including healthy and injured cells and defined genes and pathways that help recovery or predict decline in kidney function.