Human iPSC Core

The induced pluripotent stem cells (iPSC) core has set up an infrastructure of inter-institutional regulatory approvals to provide a number of human iPSC cell lines including parent and lineage and cell type specific reporter lines that can be differentiated into various kidney cell types.  These cell lines are distributed throughout the world for various aspects of kidney development, disease modelling and tissue engineering.

Summary of iPSC lines being distributed

no.	Gene	Reporter	Targeting approach	Purpose	Parental line	Target validation	Genome integrity
1	Control	NA	NA	Parental	BJFF6	Y	Y
2	Control	NA	NA	Parental	AN1	Y	Y
3	Control	NA	NA	Parental	WTC11	Y	Y
4	RET:AAVS1	tdT:GCaMP6f	5′, T2A	Collecting duct progenitors, UB:calcium sensor	WTC11	Y	Y
5	WNT9B	mCerulean3	3′, T2A	UB stalk	BJFF6	Y	Y
6	CA2	E2 Crimson	3′, T2A	Intercalated cells	BJFF6	Y	Y
7	AQP2	EGFP	3′, T2A	Principal cells	BJFF6	Y	Y
8	UPK3A	mCerulean3	5′, T2A	Ureter/bladder epithelium	BJFF6	Y	Y
9	HCN3	membranetdT	3′, T2A	Pacemaker cells	BJFF6	Y	Y
10	SIX2	EGFP	3′, T2A	nephron progenitors	CRL-2429	Y	Y
11	CITED1	mCherry	3, T2A	nephron progenitors	CRL-2429	Y	Y
12	GATA3	mCherry	5′, T2A	collecting duct	CRL-2429	Y	Y
13	CUBN	EGFP	5′, polyA	proximal tubule	CRL-2429	Y	Y
14	MAFB	mTagBFP2	5′, polyA	podocytes	CRL-2429	Y	Y
15	SIX2/ CITED1	EGFP/ mCherry	As above	nephron progenitors	CRL-2429	Y	Y
16	GATA3/ MAFB	mCherry/ mTagFBP2	As above	collecting duct and podocytes	CRL-2429	Y	Y
17	GAPDH	EGFPloxP mCherry	3′, T2A	Lineage tracing tool	CRL-2429	Y	Y
18	GAPDH/ SIX2	EGFPloxP mCherry/ Cre	3′, T2A 3, T2A	lineage tracing of nephron progenitors	CRL-2429	Y	Y
19	GATA3/ CUBN/ MAFB	mCherry/ EGFP/ mTagBFP2	As above	collecting duct, proximal tubule and podocytes	CRL-2429	Y	Y
20
21	GAPDH/ SIX2	EGFPloxP mCherry/ CreERT2	3′, T2A 3, T2A	Lineage tracing of nephron progenitors	CRL-2429	Y	Y

Director