Human iPSC Core

The induced pluripotent stem cells (iPSC) core has set up an infrastructure of inter-institutional regulatory approvals to provide a number of human iPSC cell lines including parent and lineage and cell type specific reporter lines that can be differentiated into various kidney cell types.  These cell lines are distributed throughout the world for various aspects of kidney development, disease modelling and tissue engineering.

The details of how to request cell lines are provided at (Re)Building a Kidney consortium.


Summary of iPSC lines being distributed

no.GeneReporterTargeting approachPurposeParental lineTarget validationGenome integrity
1ControlNANAParentalBJFF6YY
2ControlNANAParentalAN1YY
3ControlNANAParentalWTC11YY
4RET:AAVS1tdT:GCaMP6f5′, T2ACollecting duct progenitors, UB:calcium sensorWTC11YY
5WNT9BmCerulean33′, T2AUB stalkBJFF6YY
6CA2E2 Crimson3′, T2AIntercalated cellsBJFF6YY
7AQP2EGFP3′, T2APrincipal cellsBJFF6YY
8UPK3AmCerulean35′, T2AUreter/bladder epitheliumBJFF6YY
9HCN3membranetdT3′, T2APacemaker cellsBJFF6YY
10SIX2EGFP3′, T2Anephron progenitorsCRL-2429YY
11CITED1mCherry3, T2Anephron progenitorsCRL-2429YY
12GATA3mCherry5′, T2Acollecting ductCRL-2429YY
13CUBNEGFP5′, polyAproximal tubuleCRL-2429YY
14MAFBmTagBFP25′, polyApodocytesCRL-2429YY
15SIX2/
CITED1
EGFP/
mCherry
As abovenephron progenitors CRL-2429YY
16GATA3/
MAFB
mCherry/
mTagFBP2
As abovecollecting duct and podocytesCRL-2429YY
17GAPDHEGFPloxP mCherry3′, T2ALineage tracing toolCRL-2429YY
18GAPDH/
SIX2
EGFPloxP mCherry/
Cre
3′, T2A
3, T2A
lineage tracing of nephron progenitorsCRL-2429YY
19GATA3/
CUBN/
MAFB
mCherry/
EGFP/
mTagBFP2
As abovecollecting duct, proximal tubule and podocytesCRL-2429YY
20
21GAPDH/
SIX2
EGFPloxP mCherry/
CreERT2
3′, T2A
3, T2A
Lineage tracing of nephron progenitorsCRL-2429YY
Lines 1-9: Jain / 10-21: Little through RBK

Director