Human iPSC Core
The induced pluripotent stem cells (iPSC) core has set up an infrastructure of inter-institutional regulatory approvals to provide a number of human iPSC cell lines including parent and lineage and cell type specific reporter lines that can be differentiated into various kidney cell types. These cell lines are distributed throughout the world for various aspects of kidney development, disease modelling and tissue engineering.
The details of how to request cell lines are provided at (Re)Building a Kidney consortium.
Summary of iPSC lines being distributed
no. | Gene | Reporter | Targeting approach | Purpose | Parental line | Target validation | Genome integrity |
---|---|---|---|---|---|---|---|
1 | Control | NA | NA | Parental | BJFF6 | Y | Y |
2 | Control | NA | NA | Parental | AN1 | Y | Y |
3 | Control | NA | NA | Parental | WTC11 | Y | Y |
4 | RET:AAVS1 | tdT:GCaMP6f | 5′, T2A | Collecting duct progenitors, UB:calcium sensor | WTC11 | Y | Y |
5 | WNT9B | mCerulean3 | 3′, T2A | UB stalk | BJFF6 | Y | Y |
6 | CA2 | E2 Crimson | 3′, T2A | Intercalated cells | BJFF6 | Y | Y |
7 | AQP2 | EGFP | 3′, T2A | Principal cells | BJFF6 | Y | Y |
8 | UPK3A | mCerulean3 | 5′, T2A | Ureter/bladder epithelium | BJFF6 | Y | Y |
9 | HCN3 | membranetdT | 3′, T2A | Pacemaker cells | BJFF6 | Y | Y |
10 | SIX2 | EGFP | 3′, T2A | nephron progenitors | CRL-2429 | Y | Y |
11 | CITED1 | mCherry | 3, T2A | nephron progenitors | CRL-2429 | Y | Y |
12 | GATA3 | mCherry | 5′, T2A | collecting duct | CRL-2429 | Y | Y |
13 | CUBN | EGFP | 5′, polyA | proximal tubule | CRL-2429 | Y | Y |
14 | MAFB | mTagBFP2 | 5′, polyA | podocytes | CRL-2429 | Y | Y |
15 | SIX2/ CITED1 | EGFP/ mCherry | As above | nephron progenitors | CRL-2429 | Y | Y |
16 | GATA3/ MAFB | mCherry/ mTagFBP2 | As above | collecting duct and podocytes | CRL-2429 | Y | Y |
17 | GAPDH | EGFPloxP mCherry | 3′, T2A | Lineage tracing tool | CRL-2429 | Y | Y |
18 | GAPDH/ SIX2 | EGFPloxP mCherry/ Cre | 3′, T2A 3, T2A | lineage tracing of nephron progenitors | CRL-2429 | Y | Y |
19 | GATA3/ CUBN/ MAFB | mCherry/ EGFP/ mTagBFP2 | As above | collecting duct, proximal tubule and podocytes | CRL-2429 | Y | Y |
20 | |||||||
21 | GAPDH/ SIX2 | EGFPloxP mCherry/ CreERT2 | 3′, T2A 3, T2A | Lineage tracing of nephron progenitors | CRL-2429 | Y | Y |