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Human iPSC Core

The induced pluripotent stem cells (iPSC) core has set up an infrastructure of inter-institutional regulatory approvals to provide a number of human iPSC cell lines including parent and lineage and cell type specific reporter lines that can be differentiated into various kidney cell types. These cell lines are distributed throughout the world for various aspects of kidney development, disease modelling and tissue engineering.

A Just-In-Time (JIT) funding opportunity is available to subsidize services from this core. Apply now.

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Pediatric Kidney Tissue Core

Pediatric kidney tissue will be preserved in different media for broad application in single cell omics technologies.  Planned preservations include FFPE blocks, Fresh frozen OCT-embedded blocks, flash frozen tissue, cryoprotected fixed frozen OCT-embedded tissue blocks and parent and reporter human iPSC lines. The source of pediatric deceased donor kidney tissue is from a network of organ procurement centers coordinated by Gloria Pryhuber, MD at University of Rochester Medical Center and nephrectomy and biopsy cases at Washington University coordinated by Sanjay Jain, MD, PhD. As the repository is being built, we anticipate pediatric kidney tissue to be available upon request and necessary regulatory approvals in 2024.

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Single Cell & Spatial Biology Core

Accelerating pediatric kidney disease research

This core is directed by Drs. Sanjay Jain and Michael Eadon. The PCEN investigators are leaders in the field of single cell and spatial biology with expertise ranging from tissue acquisition, processing, data generation and analytics. They have generated a wealth of multiomics data from human kidneys. The services here will provide resources to accelerate pediatric kidney disease research and focused support for queries of genes and potentially wet lab support for conducting spatial biology experiments.

  • Voucher support to accelerate pediatric kidney disease research by using GTAC@MGI core services for various single cell and spatial assays. Apply now.
  • Providing cell type identity and spatial expression using existing kidney atlas. Request service.

Coming Summer 2024: Spatial transcriptomics experiment using Xenium or CosMx, fee for service structure for us to run the experiment.