The induced pluripotent stem cells (iPSC) core has set up an infrastructure of inter-institutional regulatory approvals to provide a number of human iPSC cell lines including parent and lineage and cell type specific reporter lines that can be differentiated into various kidney cell types. These cell lines are distributed throughout the world for various aspects of kidney development, disease modelling and tissue engineering.
Pediatric kidney tissue will be preserved in different media for broad application in single cell omics technologies. Planned preservations include FFPE blocks, Fresh frozen OCT-embedded blocks, flash frozen tissue, cryoprotected fixed frozen OCT-embedded tissue blocks and parent and reporter human iPSC lines. The source of pediatric deceased donor kidney tissue is from a network of organ procurement centers coordinated by Gloria Pryhuber, MD at University of Rochester Medical Center and nephrectomy and biopsy cases at Washington University coordinated by Sanjay Jain, MD, PhD. As the repository is being built, we anticipate pediatric kidney tissue to be available upon request and necessary regulatory approvals in 2024.