Project 1
Project 1 of pKidCAP will construct a multimodal atlas of spatially resolved single nucleus gene expression and chromatin state maps with accompanying genomic variants at key stages of pediatric kidney development by using unique and innovatively collected high quality pediatric kidney samples from the Biomedical Core (pKidBIO).
Aim 1: To define the cellular diversity, gene expression and epigenetic landscape (from the same cell), associated molecular trajectories mapped to histological space using spatial transcriptomics and DNA variants in human reference kidneys across the pediatric lifespan at timepoints relevant to kidney function and disease.
Aim 2: To define the gene expression and epigenetic landscape (from same cell) in kidney biopsies from children with glomerular disease with a focus on focal segmental glomerulosclerosis (FSGS) and identify genes and pathways associated with shifts from healthy to altered cell states.