Pediatric kidney tissue will be preserved in different media for broad application in single cell omics technologies. Planned preservations include FFPE blocks, Fresh frozen OCT-embedded blocks, flash frozen tissue, cryoprotected fixed frozen OCT-embedded tissue blocks and parent and reporter human iPSC lines. The source of pediatric deceased donor kidney tissue is from a network of organ procurement centers coordinated by Gloria Pryhuber, MD at University of Rochester Medical Center and nephrectomy and biopsy cases at Washington University coordinated by Sanjay Jain, MD, PhD. As the repository is being built, we anticipate pediatric kidney tissue to be available upon request and necessary regulatory approvals in 2024.
Professor of Pediatrics, Neonatology
University of Rochester Medical Center, Rochester, New York
Gloria Pryhuber is a physician-scientist, currently Professor of Pediatrics (Neonatology) and Environmental Medicine at the University of Rochester Trained at the University for Cincinnati, Cincinnati Children’s Hospital Medical Center Pulmonary Biology Division. She has actively studied pathogenesis of chronic lung disease, especially following premature birth, for more than 25 years. In addition to leadership in clinical-translational studies, she is the PI for Phase I and Phase II of the Lung Development Molecular Atlas Program Human Tissue Core (LungMAP HTC) and now the HuBMAP-Lung Tissue Mapping Center. In partnership with the UNOS national transplant network, using low post-mortem interval, transplant organ recovery protocols, she created and manages the unique BioRepository for Investigation of Neonatal Diseases of the Lung (BRINDL), containing consented, transplant-quality, biospecimens of pediatric research tissues including trachea and lung tissue, peripheral blood mononuclear cells (PBMC), lymph node, spleen and thymus, and has provided over 40 research laboratories with unique opportunities to explore the developing human respiratory tract and immune system in an unusually holistic manner. HTC BRINDL samples have been used and published, by Pryhuber’s laboratory and by collaborators, for flow and mass cytometry, single cell and nuclear transcriptomics, epigenomics, unbiased mass spectrometry lipid and protein identification, highly multiplexed immunofluorescence and spatial transcriptomics. Most recently, in collaboration with Sanjay Jain, MD, PhD, she brings this infrastructure and capability to the PCEN pKidBIO Biospecimens Core for biobanking of pediatric human kidney, ureter and bladder, enthusiastic to support an outstanding team of scientists, students and clinical subspecialists in urology and nephrology.